SCIENCE: A cutting-edge microscope unique in France installed on the Inrae site in Dijon

For its tenth anniversary, the Burgundy-Franche-Comté cell imaging platform DImaCell received as a gift a new transmission electron microscope allowing cells to be seen inside cells with a resolution of the order of a nanometer.

In November 2020, the regional cell imaging platform DImaCell celebrated its tenth anniversary. As a birthday present, she was given a new Transmission Electron Microscope (TEM) unique in France and “capable of pushing back the boundaries of observation of living things” according to Inrae (National Research Institute for agriculture, food and the environment).

The new MET was installed on the Dijon site of the Inrae Bourgogne-Franhce-Comté center. The investment represents nearly one million euros. It was funded by the Bourgogne-Franche-Comté regional council, the state, and by the DImaCell platform itself. The new tool complements the equipment at this center, which already comprises a confocal laser scanning microscope and a transmission and scanning electron microscope.

A very high resolution camera, the first installed in France

Twenty years separate this last microscope from its predecessor on the platform. The result of technological developments in the field of microscopy, this MET considerably broadens the imaging possibilities available to the scientific community in biology at the Federal University of Bourgogne-Franche-Comté: cryo-microscopy, 3D tomography, chemical analysis , Correlative Light and Electron Microscopy.

This new MET is equipped with a very high resolution AMT nanosprint 43 camera. Its configuration in Dijon, unique at the national level, places the device at the forefront of microscopy equipment currently available in Europe. This equipment strengthens the equipment pool and the methodological panel of the DImaCell platform, whose expertise in cellular and molecular imaging, in situ or in vivo, is already recognized by the scientific community.

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Ten years of progress in microscopy in Bourgogne-Franche-Comté

The DImaCell platform, or Cellular Imaging Device, was created in 2009 to structure the skills and know-how of universities and research institutions in Bourgogne-Franche-Comté in the field of cell imaging.

DImaCell’s equipment is spread over three technical platforms: photon microscopy (Agrosup Dijon site, INRAE ​​Dijon site and UFC Santé Besançon site), electronic imaging (INRAE ​​Dijon site and PF ARCEN Dijon), laser microdissection (UFC Santé Besançon site and INRAE ​​site Dijon). The platform’s regular investment in equipment provides its partners with the latest generation of services.

The platform’s high-level technologies and methodologies in the field of cell imaging are made available to the entire scientific community of the Bourgogne-Franche-Comté region (universities, public scientific and technological institutions and companies private).

Original expertise in plant health

The platform is spread over two sites on the Dijon campus (INRAE ​​and AgroSup) as well as one site on the Besançon campus (UFC santé). It allows any user to carry out a complete cell imaging project within a unique technological environment, ensuring both sample preparation and microscopic observations. The user thus benefits from a set of skills, equipment, techniques, preparation methods and services for the development of innovative methods, which are made available to him and on which he can be trained.

The INRAE ​​site of the DImaCell platform is backed by the joint Agroecology research unit. The site offers partners multiscale and multidisciplinary approaches with original expertise in plant health, in understanding the molecular mechanisms underlying plant / microorganism interactions (mutualistic or pathogenic fungi or bacteria), plant immunity and membrane characterization. plant cells.

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The site has high-level microscopy instrumentation for cryo-methods (complete set of preparation and observation of vitrified biological samples), optical microscopy technologies with particular expertise in plant imaging and quantitative image analysis. or data.

Jean-Christophe Tardivon

The highlights of the Hitachi H7800 transmission electron microscope – 120kV

Fully digital
Tomography for 3D imaging
EDX microanalysis module for elementary imaging of biological material
Two CMOS technology cameras including a very high resolution AMT nanosprint43 camera, the first installed in France
Cryogenic stage for cryomicroscopy
Mirror-Clem module to interface light microscopy and electron microscopy (correlative microscopy)
Ergonomics of work
Energy saving

Find a presentation of the MET H7800 Hitachi in a video from Milexia France

The Hitachi H7800 Transmission Electron Microscope – 120kV

Bacteriophage virus

Yeasts from the vineyard
(DImaCell visuals)

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