Here’s a breakdown of the flow cytometry panel used in the study, extracted from the provided text:
Flow Cytometry Panel:
The study used flow cytometry to analyze circulating T cells. The following antibodies were used, each with its respective catalog number, clone, and volume used per 100 μl of blood:
* CD3: BV510 (cat. no. 563109, clone UCHT1)
* CD4: APC-Cy7 (cat. no. 557922,clone RPA-T4)
* CD8: BV711 (cat. no. 563677,clone RPA-T8)
* CD45RA: APC-H7 (cat. no. 560674, clone HI100)
* CD183 (CXCR3): BV421 (cat. no. 562558, clone 1C6/CXCR3)
* CD127 (IL-7Rα): BV605 (cat. no. 562662, clone HIL-7R-M21)
* CD279 (PD-1): BV786 (cat. no. 563789, clone EH12.1)
Purpose:
These markers were used to characterize T regulatory cells (Treg cells) and T effector cells (Teff cells) in the circulation. The combination of these markers allows for the identification and phenotyping of different T cell subsets.
