Low Oxygen Culturing Boosts Stem cell Potential for Ovarian Failure Treatment, New Research Shows
Jakarta, Indonesia – A new study from researchers at[Institution-[Institution-[Institution-[Institution-not specified in provided text, but implied to be Indonesian based on author affiliations]demonstrates that culturing mesenchymal stem cells (MSCs) under low oxygen conditions substantially enhances their viability and potential for treating ovarian failure. The findings, published in F1000Research, offer a promising strategy to overcome a major hurdle in stem cell transplantation therapies.
Stem cell transplantation has been investigated as a potential treatment for a range of degenerative diseases, including stroke, Alzheimer’s, diabetes, Parkinson’s, and ovarian failure – a condition impacting female reproductive health by disrupting egg production and hormone balance. mscs,derived from sources like bone marrow,adipose tissue,and cord blood,have shown initial promise in repairing damaged tissues and promoting the advancement of germinal cells.
However, previous attempts at MSC transplantation have been hampered by low cell survival rates and limited adaptability to the recipient’s body. A key factor contributing to this is apoptosis – programmed cell death – occurring both during cell culture and after transplantation. This necessitates high doses of cells and frequent boosters, driving up treatment costs.
The research team, led by Erma Safitri, Hery Purnobasuki, Muhammad Thohawi Elziyad Purnama, and Shekhar Chhetri, investigated whether mimicking the low oxygen habitat of the bone marrow niche could improve MSC performance. Their in vitro studies revealed that culturing MSCs under low oxygen pressure increased their biological properties.
Specifically,the study focused on the expression of key proteins: HSP70 and Caspase-3,indicators of apoptosis,and VEGF-1 and GDF-9,markers of cell viability and differentiation. Results showed that low oxygen pressure reduced the expression of HSP70 and Caspase-3, suggesting decreased apoptosis, while increasing the expression of VEGF-1 and GDF-9, promoting cell survival and development.
The researchers conducted their experiments using female mice experiencing ovarian failure induced by malnutrition. The findings suggest that low oxygen culture could be a valuable technique for enhancing MSC maintenance and improving the effectiveness of stem cell therapies for ovarian failure by promoting folikulogenesis and oogenesis – the development of follicles and egg cells, respectively. The activation of HSP27 through Caspase-9 suppression, observed in previous research on low oxygen cultivation, may also contribute to the anti-apoptotic effects.
