Here’s a breakdown of the references cited in the provided text, organized for clarity:
* 29: Morfopoulou, S. et al. Genomic investigations of unexplained acute hepatitis in children.Nature 617, 564–573 (2023). (Cited multiple times – related to AAV2 and HHV-6 in hepatitis cases)
* 30: Ho, A. et al. Adeno-associated virus 2 infection in children with non-A–E hepatitis. Nature 617, 555–563 (2023). (Related to AAV2 and hepatitis)
* 36: Biotechnol. 42, 1232–1242 (2024). (Reference is incomplete, but likely related to AAV vector structures)
* 41: Sun, X. et al. Molecular analysis of vector genome structures after liver transduction by conventional and self-complementary adeno-associated viral serotype vectors in murine and nonhuman primate models.Hum. Gene Ther. 21, 750–761 (2010).(Related to AAV genome structures in liver cells)
* 42: Dalwadi, D. A. et al. AAV integration in human hepatocytes.Mol. Ther. 29, 2898–2909 (2021). (Related to AAV integration in human liver cells)
* 43: Warner,S., Brown, R. M., Reynolds, G. M., Stamataki, Z. & Kelly, D. A. Case report: acute liver failure in children and the human herpes virus 6-? A factor in the recent epidemic. Front Pediatr. 11, 1143051 (2023). (related to HHV-6 and acute liver failure)
* 44: Yang, C.H. et al. Evaluating for human herpesvirus 6 in the liver explants of children with liver failure of unknown etiology. J. Infect. Dis. 220, 361–369 (2019). (Related to HHV-6 in liver failure)
* 45: Allen, J. M., Debelak, D. J., Reynolds, T. C. & Miller, A. D. Identification and elimination of replication-competent adeno-associated virus (AAV) that can arise by nonhomologous recombination during AAV vector production. J. Virol. 71, 6816–6822 (1997). (Related to replication-competent AAV formation during production)
* 46: Yang, J. et al. Concatamerization of adeno-associated virus circular genomes occurs through intermolecular recombination. J. Virol. 73, 9468–9477 (1999). (Related to AAV genome concatemerization)
* 47: Yan, Z., zak, R., Zhang, Y. & Engelhardt, J.F.Inverted terminal repeat sequences are significant for intermolecular recombination and circularization of adeno-associated virus genomes. J. Virol. 79, 364–379 (2005).(Related to AAV genome recombination and circularization)
Key Themes of the References:
* AAV Genome structure & Integration: Several references (41,42,46,47) focus on how AAV genomes behave within cells – how they form structures like concatemers,and whether/how they integrate into the host genome.
* AAV & Hepatitis: References 29 and 30 link AAV2 (wild-type) to cases of unexplained hepatitis in children.
* HHV-6 & Liver Disease: References 29, 43, and 44 explore the presence of HHV-6 in liver tissue, especially in cases of hepatitis or liver failure.
* AAV Production & Safety: Reference 45 addresses the potential for creating replication-competent AAV during the manufacturing process, a safety concern.