single-Cell Analysis Targets Selective Splicing in Alzheimer’s, Parkinson’s

in a groundbreaking effort to understand the complexities of neurodegenerative diseases, researchers at Mount sinai’s Icahn School of Medicine, with support from Parse Biosciences, are embarking on a project to analyze selective splicing events at the single-cell level. The study will focus on Alzheimer’s and Parkinson’s disease, aiming to uncover new insights into disease mechanisms and potential therapeutic targets.This initiative will analyze transcriptome maps of over 10 million cells from more then 1,000 samples of peripheral blood mononuclear cells (PBMCs) from patients with neurodegenerative diseases.

Unlocking the Secrets of Selective Splicing

Conventional research often emphasizes the genomic origins of neurodegenerative diseases.However, this new study shifts focus to post-transcriptional events, specifically selective splicing, which may considerably influence disease pathology and progression. Selective splicing is a crucial process that allows a single gene to code for multiple proteins,adding another layer of complexity to cellular function [1].

Did You Know? Choice splicing is estimated to occur in approximately 95% of human multi-exonic genes, highlighting its importance in regulating gene expression [2].

By observing selective splicing events in individual cells on a large scale, researchers hope to identify factors that influence the onset, progression, and severity of Alzheimer’s, Parkinson’s, and amyotrophic lateral sclerosis (ALS). Dr. Raj’s research team at Mount Sinai has a long-standing commitment to unraveling the molecular mechanisms of these debilitating conditions.

High-throughput Analysis with Cutting-Edge technology

The Mount Sinai team will conduct high-throughput analysis of PBMC samples from confirmed alzheimer’s and parkinson’s patients. These samples will then be sent to Parse GigaLab, a specialized facility equipped for generating large-scale single-cell RNA sequencing data sets. Parse Biosciences’ Evercode chemistry enables GigaLab to rapidly produce high-quality, extensive single-cell datasets.

Pro Tip: Single-cell RNA sequencing (scRNA-seq) allows researchers to examine gene expression in individual cells, providing a much more detailed understanding of cellular heterogeneity than traditional bulk sequencing methods [3].

The collaboration between Mount Sinai and Parse Biosciences leverages advanced technology to accelerate the research process and gain unprecedented insights into neurodegenerative diseases. The data generated is expected to establish a fundamental model that will improve our understanding, detection, and treatment of these conditions.

Parse Biosciences: Advancing Single-Cell Sequencing

Parse Biosciences, a global biosciences company, is dedicated to accelerating progress in human health and scientific research by making single-cell sequencing more accessible and convenient. Their technology has contributed to breakthroughs in various fields, including cancer treatment, tissue repair, and stem cell therapy.Founded on technology developed at the university of Washington, Parse Biosciences has secured over $100 million in funding and serves more than 2,500 customers worldwide.

Key Project Metrics

The Future of neurodegenerative Disease Research

This ambitious project represents a significant step forward in understanding the intricate molecular mechanisms underlying neurodegenerative diseases. By focusing on selective splicing and utilizing cutting-edge single-cell analysis techniques, researchers are paving the way for new diagnostic and therapeutic strategies.

What other post-transcriptional modifications might play a role in neurodegenerative diseases? How can these findings be translated into effective treatments for patients suffering from Alzheimer’s and Parkinson’s?

Disclaimer: This article provides data for general knowledge and informational purposes only,and does not constitute medical advice.It is essential to consult with a qualified healthcare professional for any health concerns or before making any decisions related to your health or treatment.

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